Alcian BlueGlycosaminoglycans- Polysaccharides stain blue
- Nuclei stain red
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Astra Blue – Vital New RedMast cells, eosinophils- Mast cells stain blue, eosinophils stain red
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Chloroacetate EsteraseEsterase activity in mast cells- Esterase activity stain red / pink
- Nuclei stain blue
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Congo RedAmyloid- Eosinophils and elastin stain brick red
- Nuclei stain blue
- Other tissue elements stain clear / pale pink
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GiemsaBlood cells and parasites- Nuclei stain blue / purple
- Acidophils stain pink / red
- Basophils stain blue
- Eosinophils stain red / orange
- Mast cells stain purple (granules)
- Parasites stain blue / dark blue
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Goldner’s TrichromeOsteoid- Mineralized bone stain green
- Osteoid stain orange / red
- Cartilage stain purple
- Nuclei stain blue
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Masson’s TrichromeConnective tissue- Collagen fibers stain blue
- Nuclei stain black
- Cytoplasm
- Keratin
- Muscle fibers
- Intracellular fibers stain red
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Movat PentachromeElastic fibres, mucin, and collagen- Elastic fibres stain purple / black
- Muscle stains red
- Collagen and bone stains yellow
- Mucicarmine stain
- Ground substance stains blue / green
- Cytoplasm stains pink / brown
- Nuclei stains dark purple / black
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Myosin ATPase 4.2, 4.6, 9.4Muscle fibre types- Muscle fibre type 1, 2A, 2B, and 2C demonstrated in varying shades of grey to black
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Oil-Red-OFats- Lipids stain bright red
- nuclei stain blue
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PASCarbohydrates, glycogen, mucin, glycoprotein, and some basement membranes- stains magenta
- Nuclei stain blue
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Phloroglucinal stainPlant stain- Lignin in plant cell wall stain red
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Picrosirius redCollagen- Collagen stain red
- Nuclei stain grey / black
- Other tissue elements stain pale yellow
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Silver StainNeurons, glial cells, and reticular fibers | Click to enlarge |
Toluidine BlueMast cells- Stains red
- Other tissue elements stain varying shades of blue
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TUNELCellular apoptosis- Apoptotic cells stain brown / black
- Nuclei stain light blue
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Verhoeff’s Van GiesonConnective tissue- Elastic fibers and nuclei stain black
- Collagen stains red
- Other tissue elements stain yellow
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Wright’s StainBlood film- Erythrocytes stain yellow / red
- Polymorphonuclear neutrophils’ cytoplasm stain pink / tan
- Eosinophils’ granules stain red / orange; basophils’ granules stain purple
- Lymphocytes’ cytoplasm stain sky blue
- Nuclei stain reddish purple
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