Resin Histology

 
 

Histological evaluation is the preferred method for examining the in-situ interaction between implanted medical devices and its surrounding tissue. The challenge lies in maintaining the interaction between the device and tissue due to differences in density. This method will present a similar density between the materials, allowing for a clean section through the tissue, device, and the interface. We have specially developed protocols to process, section, and stain devices implanted in tissue.

With this method, it is possible to examine the changes taking place at the interaction site such as scar tissue formation, changes to tissue architecture, changes in collagen and other protein profiles, tissue infiltration into the device and inflammation. Since most medical devices differ in material, our research team will work closely with you to develop specific protocols for your studies. In general, softer devices can be sectioned and deplastinated to obtain the best staining results.

Cutting & Grinding

For harder devices, the cutting & grinding technique is used to obtain 10μm section the best resolution. This sectioning method consist of a precision diamond blade saw and a semi-automated grinder/polisher. The saw is used to cut a think ‘wafer’ out of the resin-embedded tissue block then glued onto a slide. From there it is ground and polished to the required thickness.

Medical Device Tissue Interaction Analysis

The image below highlights surrounding tissue that is interacting with a bare-metal stent, embedded within the arterial wall. Using high powered imaging software, customized reports can be generated to score for pathological analysis.

The research areas we serve include but are not limited to the following:

  • Soft and hard tissues, implanted medical devices and bone
  • Custom protocol development for each device type
  • Microtome sectioning for softer devices
  • Cutting & Grinding for harder devices up to 2″ x 3″ and 1″ x 3″ slide size
  • Example of devices: stents, artificial muscle, heart valves, silk and artificial fibres, bio-absorptive materials, sutures, and polymers
  • Full pathology report by U.S. Board Certified Pathologist

View Wax-it’s Medical Device Stain Gallery

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