Immunohistochemistry

IHC is the process of localizing proteins in cells of a tissue section by exploiting the principle of antibodies binding to specific antigens. Due to the complex nature of antigens and their corresponding antibodies, protocols for IHC vary widely. At Wax-it, we work with you to efficiently develop protocols, and identify biomarkers/antibodies of interest to bring you results of the highest quality. Researchers can choose from a variety of IHC services to meet their study objectives.

Antibody Testing & Validation

Wax-it Histology Services provides antibody testing and antibody validation services to help our clients reach their research milestones. At Wax-it Histology Services, we work with you to test and validate antibodies that have not previously been tested for use in immunohistochemistry (IHC) applications. We follow stringent antibody validation protocols to ensure credible and reproducible results, conserving your valuable time and resources.

Tissue Bank

As a complement to our Immunohistochemistry (IHC) Services, we have full access to an in-house Tissue Bank and Tissue MicroArray bank. Our in-house Tissue Bank – a collection of tissues from multiple species including mouse, rat, pig, rabbit, and monkey – allows us to identify proteins of interest in multiple tissues on a single slide, providing a truly macroscopic view of protein distribution, and a complete antibody cross-reactivity profile. Our in-house Tissue MicroArray system permits rapid screening of potential tissue cross-reactivity. These Tissue MicroArrays incorporate multiple arrayed tissues from the same species into a single tissue block, which significantly reduces costs (as multiple tissues are stained on a single slide).

  • Access to tissue bank
  • Background literature research
  • Sourcing of optimal antibodies
  • Antibody cross-reactivity
  • Antibody testing & validation
  • Protocol development
  • Routine IHC staining (manual and automated)
  • Slide analysis, including digital microscopy imaging, digital whole slide scanning, staining quantification, pathological scoring/analysis, and report generation