Conducting an IHC Study - Factors to Consider: Part II
Immunohistochemistry (IHC) is a method for demonstrating the presence and location of proteins in tissue. IHC enables the observation of processes in the context of intact tissue, which is especially useful for assessing the progression and treatment of certain diseases. Immunohistochemical staining is accomplished with antibodies that recognize the target protein. Since antibodies are highly specific, the antibody will only bind to the protein of interest. The antibody and antigen interaction is then visualized using chromogenic detection.
Finding an Appropriate Primary Antibody
As discussed in the article “Conducting an IHC Study - Factors to Consider: Part 1”, when conducting immunohistochemistry, one must first consider the target of interest, availability of primary antibodies, type of sample processing and detection system, protocol development, and antibody interpretation; Once these steps are complete, identify the appropriate primary antibody while considering the following factors:
More Articles
- Advantages and Drawbacks of Paraffin and Frozen Tissues
- Fixation and Shipment of Tissue Samples for Frozen Downstream Applications
- Fixation and Shipment of Wet Tissues for Paraffin Embedding Downstream Services
- Why More Organizations are Outsourcing Histopathology
- Histopathology for Oncology Therapeutics
- Resin Histology by Device Type (Cut & Grind vs. Microtomy)
- Histopathological Considerations for IHC-based Studies
- Fixing Tissue for Optimal Results: Part II
- Fixing Tissue for Optimal Results: Part 1
- Conducting an IHC Study - Factors to Consider: Part II
- Conducting an IHC Study - Factors to Consider: Part 1
- The Histological Analysis of Implantable Medical Devices
- Conducting Biocompatibility Testing for a Medical Device: Part II
- Conducting Biocompatibility Testing for a Medical Device: Part I
- Challenges Faced by Medical Device Companies: Part II
- Challenges Faced by Medical Device Companies: Part I
- Biocompatibility of Implants/Medical Devices
Request More Information
Please provide the following information and we will email you a link to the article, "Conducting an IHC Study - Factors to Consider: Part II".
Note: * indicates a required field.