Conducting an IHC Study - Factors to Consider: Part II

Immunohistochemistry (IHC) is a method for demonstrating the presence and location of proteins in tissue. IHC enables the observation of processes in the context of intact tissue, which is especially useful for assessing the progression and treatment of certain diseases. Immunohistochemical staining is accomplished with antibodies that recognize the target protein. Since antibodies are highly specific, the antibody will only bind to the protein of interest. The antibody and antigen interaction is then visualized using chromogenic detection.

Finding an Appropriate Primary Antibody

As discussed in the article “Conducting an IHC Study - Factors to Consider: Part 1”, when conducting immunohistochemistry, one must first consider the target of interest, availability of primary antibodies, type of sample processing and detection system, protocol development, and antibody interpretation; Once these steps are complete, identify the appropriate primary antibody while considering the following factors:

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