Our Services - Antibody Cross-Reactivity
Tissue Cross Reactivity Studies
We offer both non-GLP and GLP-compliant Tissue Cross Reactivity Studies which permit researchers to investigate and validate that their antibody of interest does not bind to other epitopes other than the expected target site. Identification of such cross-reactivity in the discovery / pre-clinical stage of drug development can help to avoid adverse and unforeseen toxicological effects in future human clinical trials.
Wax-it Histology Services Inc. utilizes our in-house TissueMacroArray system to permit rapid screening of potential tissue cross-reactivity. These TissueMacroArrays incorporate multiple arrayed tissues from the same species into a single tissue block.* This provides for more consistent, reliable staining with immunohistochemistry techniques, as well as the benefit of significantly reduced costs for the sponsor as multiple tissues are stained on a single slide.
A comprehensive study program is offered to support our clients’ research needs, including:
- Complete tissue banks of human, mouse, rat, and pig
- Full medical history of human tissues
- Study-specific protocols that reflect the sponsor’s requirements
- Unparalleled expertise in immunohistochemistry, histology, immunopathology, and protocol development
- Tissue analysis conducted by board-certified pathologists
- Comprehensive, high-quality reports
- Digital photomicroscopy and/or digital whole-slide scanning
- Rapid turn-around times
- Cost-effective pricing
* TissueMacroArrays not available for human tissue, only single tissue slides
Our Process
- We first determine the optimal specificity and staining conditions for the experimental antibody. Multiple target retrieval, antibody dilutions, and appropriate positive and negative controls are used to optimize and validate immunostaining conditions.
- Based on this optimized protocol, we will perform immunostaining across 32 standard tissues, followed by evaluation and report generation. Staining of tissues can be done on an individual basis (i.e. – one tissue per slide), or by incorporating our TissueMacroArray bank into the study.
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Adrenal Gland
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Endothelium
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Ovary
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Spinal Cord
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Bladder
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Eye
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Pancreas
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Spleen
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Blood Cells
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Fallopian Tube
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Parathyroid
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Testes
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Bone Marrow
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Gastrointestinal Tract
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Pituitary
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Thymus
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Breast
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Heart
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Placenta
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Thyroid
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Cerebellum
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Kidney
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Prostate
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Ureter
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Cerebral Cortex
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Liver
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Skeletal Muscle
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Uterus
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Colon
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Lymph Node
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Skin
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- Entire data sets can be collected via digital whole-slide scanning (particularly useful when combined with our TissueMacroArrays), with all stained information being analyzed by a board-certified pathologist and corresponding report generated detailing the findings.
Wistar Male Mouse TissueMacroArray Slide Immunostained with JAG-1
PATHOLOGY REPORT
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